Introduction

The transcriptome refers to the complete collection of RNA transcripts within a particular cell during a specific developmental stage or under a defined physiological condition. A comprehension of the transcriptome is crucial for interpreting the functional components of the genome and gaining insights into the fundamental mechanisms that drive development and contribute to disease. (Yang, 2015)

The Whole Transcriptome Sequencing service offered by Genoxvision empowers researchers with advanced Next-Generation Sequencing (NGS) solutions, enabling comprehensive bioinformatic analyses of all RNA transcripts, encompassing mRNA and non-coding RNA (lncRNA, sRNA, and circRNA).

Utilizing a paired-end sequencing strategy, this service allows for accurate quantification of gene and transcript levels, identification of splice variants, and discovery of novel features within the transcriptome. This competitive approach delves into potential transcriptional and regulatory network mechanisms, offering valuable insights into interaction functionalities from a comprehensive transcriptomic standpoint.

Applications of Whole Transcriptome Sequencing

Whole Transcriptome Sequencing is utilized for various applications, including:

  • Sketching both mRNA and non-coding RNA (ncRNA) in a particular run.
  • Researching miRNA sponge and target regulatory basics.
  • Investigating regulatory networks among lncRNA/circRNA-miRNA-gene pairs. (Zeng, 2023)

Benefits of Whole Transcriptome Sequencing

  • Compared to mRNA-seq, lncRNA-seq, sRNA-seq, and circRNA-seq, Whole Transcriptome Sequencing offers a more thorough analysis of the transcriptional regulation network.
  • Researchers benefit from Whole Transcriptome Sequencing in identifying biomarkers across a wide spectrum of transcripts.
  • WTS facilitates the identification of both detected and novel features.
  • It enables comprehensive profiling of the entire transcriptome across a broad dynamic range. (Jiang,
    2015)

WTS Specifications: RNA Sample Requirements

The Workflow for the Whole Transcriptome Sequencing Service offered by the our company a systematic process:

  1. Sample Quality Control (Sample QC): The initial step involves assessing the quality of the samples to ensure they meet the criteria for RNA-Seq techniques.
  2. Library Preparation: Following Sample QC, the appropriate library is prepared based on the target organism. The prepared library undergoes testing for its quality (Library QC).
  3. Sequencing Strategy: A paired-end 150 bp sequencing strategy is employed for the lncRNA and circRNA library, while single-end 50 bp sequencing is used for the small RNA library.
  4. Data Quality Control (Data QC): The obtained data undergoes rigorous quality control procedures to guarantee the overall quality.
  5. Bioinformatic Analyses: Finally, comprehensive bioinformatic analyses are conducted, and the results are provided in a format ready for publication. The step-by-step protocol is illustrated in the following flowsheet.