Introduction

Epigenetic alterations, such as the 5-methylation of carbon in the cytosine base within a CpG dinucleotide context, play a crucial role in the initiation and advancement of human diseases. To gain a thorough insight into the impact of genome-wide DNA methylation patterns, known as the methylome, it is essential to quantitatively assess the methylation states of all CpG sites in a genome. Whole-genome bisulfite sequencing (WGBS) enables the comprehensive examination of DNA methylation across the entire genome.(Masako Suzuki, 2018)

Since its initial application in 1992 ,DNA bisulfite (BS) sequencing has emerged as the gold standard for DNA methylation analysis. The BS treatment induces the conversion of unmodified cytosines to uracil, while preserving the 5mC modification. Following PCR and sequencing, this allows for mapping at a single-base resolution. More recently, the integration of BS treatment with next-generation sequencing (NGS) has enabled the generation of reduced representation (RRBS) or whole-genome (WGBS) data, providing insights into the global genomic distribution of 5mC.(Olova, 2018)

Genoxvision offers comprehensive whole-genome bisulfite sequencing (WGBS) services, enabling the efficient identification of methylated cytosines across the entire genome with single-nucleotide resolution. The thorough exploration of the methylome is crucial for gaining insights into gene expression and various biological processes influenced by epigenetic regulation.

Applications of Whole Genome Bisulfite Sequencing

Genoxvision provides reliable data and analysis results from WGBS services, ready for publication, to support research endeavors such as:

  • Profiling methylation patterns at CG, CHG, and CHH locations with single-nucleotide resolve.
  • Recognizing differentially methylated positions associated with investigational medications or sample conditions.
  • Identifying the mechanisms underlying cell differentiation or tissue development founded on methylation outlines.
  • Allowing early diagnosis of diseases and cancers by distinguishing DNA hypermethylation or hypomethylation

Benefits of Company WGBS Service

  • We have successfully concluded numerous projects, delivering data of exceptional quality suitable for publication, along with thorough bioinformatics analysis.
  • Our methylation library preparation offers the advantage of ultra-low input DNA and a high bisulfite conversion rate.
  • Utilizing industry-standard software like Bismark and established in-house pipelines ensures precise mapping and comprehensive bioinformatics analysis.
  • Customized association analyses can investigate connections between methylation patterns and gene expression.
  • With the decreasing costs of Next-Generation Sequencing (NGS), the WGBS approach is becoming more accessible for both fundamental and clinical research (Olova, 2018)

WGBS Specifications: DNA Sample Requirements

Project Workflow of Genoxvision WGBS Service

The WGBS service offered by the Genoxvision follows a four-step process. It begins with sample preparation, progresses to library preparation, sequencing, and concludes with bioinformatics analysis. The construction of a methylation library involves DNA fragmentation and the conversion of unmethylated cytosines to uracils through sodium bisulfite treatment. Subsequently, advanced technology is used for sequencing, and the resulting reads undergo processing via bioinformatics pipelines.

To guarantee the precision and dependability of sequencing data, the company rigorously audits each experimental step through quality control measures. It ensures high-quality data output at every stage, starting from DNA sampling and extending to the generation of the final data report. The attainment of high-quality data is essential to ensure that bioinformatics analysis is accurate, thorough, and realistic.