Introduction

RNA sequencing (RNA-seq) has revolutionized the examination of cellular functionality, offering researchers unparalleled insights into the transcriptional landscape of cells. Through the utilization of high-throughput and precise next-generation sequencing (NGS) techniques, RNA-seq unveils gene expression profiles and elucidates the ongoing variations in the transcriptome. This methodology involves selectively capturing or enriching single-stranded messenger RNAs (mRNAs), which are subsequently converted into complementary DNA (cDNA) during library preparation.(Corney, 2013)

At our company, cDNA libraries undergo sequencing using cutting-edge Illumina NovaSeq platforms. These platforms employ a paired-end 150 bp sequencing strategy, focusing on short-reads. With our extensive experience and robust sequencing capacity, our company provides services tailored to fulfill diverse research objectives.

Applications of mRNA Sequencing

mRNA sequencing serves as a potent instrument for examining the cellular transcriptome profile. Genoxvision expert services contribute to the achievement of research objectives across various

  • Quantitative analysis of transcripts across diverse tissues or samples, spanning various conditions and treatments
  • Identification of new transcripts, alternative splicing (AS), and variations in transcripts
  • Exploration of developmental mechanisms and drug resistance using tissue-specific transcripts or time-course gene expression
  • Discovery of biomarkers through novel transcripts/isoforms, identification of SNP/InDel, and analysis of fusion genes
  • Integration of omics analysis with the transcriptome
  • Examination of pathogenic mechanisms and clinical subtypes for clinical diagnosis. (Kumar, 2012)

Benefits of Company mRNA Sequencing

The mRNA sequencing services provided by the Genoxvision boast high throughput and accuracy, achieving a Q30 score of ≥ 85%. This is complemented by a low initial RNA input requirement. With a wealth of experience, Genoxvision has successfully executed numerous RNA-Seq projects, assisting multiple researchers in publishing their work in high-impact factor journals.

The company provides comprehensive solutions encompassing quantification, analysis of differential gene expression, annotation of novel transcripts, exploration of alternative splicing, discovery of fusion genes, and identification of other potential variations. Skillful bioinformaticians deliver data ready for publication through customized pipelines tailored to species, whether they have a reference genome or not.

mRNA-seq Specifications: Sample Requirements

Project Workflow of Company mRNA-seq Services

The project workflow initiates with sample quality control (Sample QC) to verify that your samples adhere to RNA-Seq technique criteria. Subsequently, a suitable library is crafted based on your target organism and application, and its quality is examined (Library QC). Following that, a 150 bp paired-end sequencing strategy is employed to sequence the samples, with the resulting data undergoing quality assessment (Data QC). Finally, bioinformatic analyses are conducted, and publication-ready results are furnished. The step-by-step protocol of our mRNA-seq technique is outlined in the following flowsheet.

Sample preparation is succeeded by RNA library construction. The RNA library is generated through polyA capture (or rRNA removal) and reverse transcription of cDNA. The sample is then sequenced using Illumina PE150 technology, and the concluding step involves bioinformatics analysis.