Introduction

Chromatin immunoprecipitation followed by sequencing (ChIP–seq) is a method used to profile DNA-binding proteins, histone modifications, or nucleosomes across the entire genome. Advances in next-generation sequencing technology have significantly improved ChIP–seq, providing enhanced resolution, reduced noise, and broader coverage compared to the array-based ChIP–chip method. The declining sequencing costs have made ChIP–seq an essential tool for investigating gene regulation and understanding epigenetic mechanisms.(Park, 2009)

At our company, we provide top-tier sequencing and comprehensive bioinformatics solutions tailored for your ChIP-seq projects.

Applications

ChIP-seq addresses regulatory challenges and caters to personalized research requirements, including:

    1. Examining the regulation of genes by profiling genome-wide binding sites and analyzing motifs to understand transcription factor involvement.
    1. Mapping histone modification patterns linked to experimental treatments or sample conditions.
    1. Exploring the interplay between epigenetic profiles and transcriptional regulations through joint analysis.
    1. Conducting enhancer analysis and providing genome-wide chromatin state annotation. (Nakato, 2020)

Benefits

  • The generated data exhibits stability and reliability, ensuring high-quality sequencing with a guaranteed Q30≥ 80% sequencing score.
  • Our expert bioinformatics analysis employs industry-standard MACS2 software and utilizes the latest program version for tasks such as motif prediction, peak annotation, functional analysis, and data visualization.
  • Complementary analyses are offered to investigate connections between ChIP-seq results and gene expression.

ChIP-seq Specifications: DNA Sample Requirements

Project Workflow of Genoxvision ChIP-seq Service

The ChIP-seq service offered by the company involves four key stages: sample preparation, DNA library preparation, Illumina PE150 sequencing, and data analysis through bioinformatics pipelines. The quality and quantity of data output, crucial for subsequent bioinformatics analysis results, can be directly influenced at each step from DNA sampling to data report generation.

Stringent checks are conducted at every stage, encompassing sample quality control, library quality control, and sequencing data quality control. These measures are implemented to guarantee the sequencing data’s high quality, accuracy, and reliability, ensuring a comprehensive and reliable outcome.